abstract：:The advent of genetically modified crops in the late 1980s triggered a regulatory response to the relatively new field of plant genetic engineering. Over a 7-year period, a new regulatory framework was created, based on scientific principles that focused on risk mitigation. The process was transparent and deliberately...

journal_title：Plant biotechnology journal

authors： Smyth S,McHughen A

更新日期：2008-04-01 00:00:00

abstract：:Extracellular plant peptides perform a large variety of functions, including signalling and defence. Intracellular peptides often have physiological functions or may merely be the products of general proteolysis. Plant peptides have been identified and, in part, functionally characterized through biochemical and genet...

journal_title：Plant biotechnology journal

authors： Farrokhi N,Whitelegge JP,Brusslan JA

更新日期：2008-02-01 00:00:00

abstract：:The feasibility of using the FLP/FRT site-specific recombination system in rice for genome engineering was evaluated. Transgenic rice plants expressing the FLP recombinase were crossed with plants harbouring the kanamycin resistance gene (neomycin phosphotransferase II, nptII) flanked by FRT sites, which also served t...

journal_title：Plant biotechnology journal

authors： Hu Q,Kononowicz-Hodges H,Nelson-Vasilchik K,Viola D,Zeng P,Liu H,Kausch AP,Chandlee JM,Hodges TK,Luo H

更新日期：2008-02-01 00:00:00

abstract：:Plant chloroplasts are promising vehicles for recombinant protein production, but the process of protein folding in these organelles is not well understood in comparison with that in prokaryotic systems, such as Escherichia coli. This is particularly true for disulphide bond formation which is crucial for the biologic...

journal_title：Plant biotechnology journal

authors： Bally J,Paget E,Droux M,Job C,Job D,Dubald M

更新日期：2008-01-01 00:00:00

abstract：:Although it is one of the major crops in the world, corn has poor nutritional quality for human and animal consumption due to its low lysine content. Here, we report a method of simultaneous expression of a deregulated lysine biosynthetic enzyme, CordapA, and reduction of a bifunctional lysine degradation enzyme, lysi...

journal_title：Plant biotechnology journal

authors： Frizzi A,Huang S,Gilbertson LA,Armstrong TA,Luethy MH,Malvar TM

更新日期：2008-01-01 00:00:00

abstract：:The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

journal_title：Plant biotechnology journal

authors： Yevtushenko DP,Misra S

更新日期：2007-11-01 00:00:00

abstract：:Spike lavender (Lavandula latifolia) essential oil is widely used in the perfume, cosmetic, flavouring and pharmaceutical industries. Thus, modifications of yield and composition of this essential oil by genetic engineering should have important scientific and commercial applications. We generated transgenic spike lav...

journal_title：Plant biotechnology journal

authors： Muñoz-Bertomeu J,Sales E,Ros R,Arrillaga I,Segura J

更新日期：2007-11-01 00:00:00

abstract：:Virus-induced gene silencing (VIGS) is a powerful tool for functional analysis of genes in plants. A wide-host-range VIGS vector, which was developed based on the Cucumber mosaic virus (CMV), was tested for its ability to silence endogenous genes involved in flavonoid biosynthesis in soybean. Symptomless infection was...

journal_title：Plant biotechnology journal

authors： Nagamatsu A,Masuta C,Senda M,Matsuura H,Kasai A,Hong JS,Kitamura K,Abe J,Kanazawa A

更新日期：2007-11-01 00:00:00

abstract：:The tissue-specific expression of transgenes is essential in plant breeding programmes to avoid the fitness costs caused by constitutive expression of a target gene. However, knowledge on the molecular mechanisms of tissue-specific gene expression and practicable tissue-specific promoters is limited. In this study, we...

journal_title：Plant biotechnology journal

authors： Cai M,Wei J,Li X,Xu C,Wang S

更新日期：2007-09-01 00:00:00

abstract：:Eastern cottonwood (Populus deltoides Bartr. ex Marsh.) trees were engineered to express merA (mercuric ion reductase) and merB (organomercury lyase) transgenes in order to be used for the phytoremediation of mercury-contaminated soils. Earlier studies with Arabidopsis thaliana and Nicotiana tabacum showed that this g...

journal_title：Plant biotechnology journal

authors： Lyyra S,Meagher RB,Kim T,Heaton A,Montello P,Balish RS,Merkle SA

更新日期：2007-03-01 00:00:00

abstract：:The replacement of crude allergen extracts by selected allergens currently represents a major goal for the improvement of allergy diagnosis and immunotherapy. Indeed, the development of molecularly defined vaccines would facilitate both standardization and enhance batch-to-batch reproducibility as well as treatment sp...

journal_title：Plant biotechnology journal

authors： Lienard D,Tran Dinh O,van Oort E,Van Overtvelt L,Bonneau C,Wambre E,Bardor M,Cosette P,Didier-Laurent A,de Borne FD,Delon R,van Ree R,Moingeon P,Faye L,Gomord V

更新日期：2007-01-01 00:00:00

abstract：:The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...

journal_title：Plant biotechnology journal

authors： Beauregard M,Hefford MA

更新日期：2006-09-01 00:00:00

abstract：:To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

journal_title：Plant biotechnology journal

authors： Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

更新日期：2006-07-01 00:00:00

abstract：:DNA recombination reactions (site-specific and homologous) were monitored in the progeny of transgenic maize plants by bringing together two recombination substrates (docking sites and shuttle vectors) in the zygotes. In one combination of transgenic events, the recombination marker gene (yellow fluorescent protein ge...

journal_title：Plant biotechnology journal

authors： Djukanovic V,Orczyk W,Gao H,Sun X,Garrett N,Zhen S,Gordon-Kamm W,Barton J,Lyznik LA

更新日期：2006-05-01 00:00:00

abstract：:Single-chain variable fragments (scFvs), as well as other recombinant antibodies, have become powerful tools for therapy and analysis in both human and veterinary medicine. Transgenic plants have been developed as an efficient production system for these recombinant proteins, achieving high yields at moderate cost. Se...

journal_title：Plant biotechnology journal

authors： Scheller J,Leps M,Conrad U

更新日期：2006-03-01 00:00:00

abstract：:Recent report reviews and funding initiatives in the field of plant genomic research are considered in the context of their translation into practical and economic value via plant breeding. It is concluded that there is a deficit in investment and that a change in working styles towards knowledge sharing and connectiv...

journal_title：Plant biotechnology journal

authors： Hughes S

更新日期：2006-01-01 00:00:00

abstract：:The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence a...

journal_title：Plant biotechnology journal

authors： Waters DL,Henry RJ,Reinke RF,Fitzgerald MA

更新日期：2006-01-01 00:00:00

abstract：:A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

journal_title：Plant biotechnology journal

authors： Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

更新日期：2005-11-01 00:00:00

abstract：:The early methionine (Em) proteins are members of the late embryogenesis abundant (LEA) group of proteins that have been considered to be embryo specific. The ability of a 646-bp wheat Em promoter to control green fluorescent protein (gfp) expression was investigated in transgenic barley and rice. Seeds of transgenic ...

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ

更新日期：2005-07-01 00:00:00

abstract：:We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a t...

journal_title：Plant biotechnology journal

authors： Nanto K,Yamada-Watanabe K,Ebinuma H

更新日期：2005-03-01 00:00:00

abstract：:With the aim of increasing the methionine level in alfalfa (Medicago sativa L.) and thus improving its nutritional quality, we produced transgenic alfalfa plants that expressed the Arabidopsis cystathionine gamma-synthase (AtCGS), the enzyme that controls the synthesis of the first intermediate metabolite in the methi...

journal_title：Plant biotechnology journal

authors： Avraham T,Badani H,Galili S,Amir R

更新日期：2005-01-01 00:00:00

abstract：:Glycine betaine plays an important role in some plants, including maize, in conditions of abiotic stress, but different maize varieties vary in their capacity to accumulate glycine betaine. An elite maize inbred line DH4866 was transformed with the betA gene from Escherichia coli encoding choline dehydrogenase (EC 1.1...

journal_title：Plant biotechnology journal

authors： Quan R,Shang M,Zhang H,Zhao Y,Zhang J

更新日期：2004-11-01 00:00:00

abstract：:In this study, the effects of the accumulation of cysteine protease inhibitors on the food preferences of adult female western flower thrips, Frankliniella occidentalis (Pergande), were investigated. Representative members of the cystatin and thyropin gene families (stefin A, cystatin C, kininogen domain 3 and equista...

journal_title：Plant biotechnology journal

authors： Outchkourov NS,de Kogel WJ,Schuurman-de Bruin A,Abrahamson M,Jongsma MA

更新日期：2004-09-01 00:00:00

abstract：:Designing transformation experiments for either functional genomics or crop improvement requires knowledge of the transgene locus structure, number, transmission and expression resulting from a specific transformation method. We recently reported an improvement to the soybean [Glycine max (L.) Merrill] cotyledonary-no...

journal_title：Plant biotechnology journal

authors： Olhoft PM,Flagel LE,Somers DA

更新日期：2004-07-01 00:00:00

abstract：:In order to create a novel mechanism for herbicide resistance in plants, we expressed a single-chain antibody fragment (scFv) in tobacco with specific affinity to the auxinic herbicide picloram. Transgenic tobacco plants and seedlings expressing this scFv against picloram were protected from its effect in a dose-depen...

journal_title：Plant biotechnology journal

authors： Almquist KC,Niu Y,McLean MD,Mena FL,Yau KY,Brown K,Brandle JE,Hall JC

更新日期：2004-05-01 00:00:00

abstract：:A high throughput proton nuclear magnetic resonance spectroscopy method for the metabolite fingerprinting of plants was applied to genetically modified peas (Pisum sativum) to determine whether biochemical changes, so called 'unintended effects', beyond those intended by incorporation of a transgene, were detectable. ...

journal_title：Plant biotechnology journal

authors： Charlton A,Allnutt T,Holmes S,Chisholm J,Bean S,Ellis N,Mullineaux P,Oehlschlager S

更新日期：2004-01-01 00:00:00

abstract：:Transgenic rice expressing soybean glycinin in its endosperm was crossed with two types of low-glutelin mutants to determine how much storage the protein mutants can contribute to increases in glycinin accumulation. The glycinin level (102 microg/100 mg seed) in the parental transgenic line was enhanced to approximate...

journal_title：Plant biotechnology journal

authors： Tada Y,Utsumi S,Takaiwa F

更新日期：2003-11-01 00:00:00

abstract：:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...

journal_title：Plant biotechnology journal

authors： Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJ

更新日期：2003-05-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:Expression of Bacillus thuringiensis delta-endotoxins has proven to be a successful strategy for obtaining insect resistance in transgenic plants. Drawbacks of expression of a single resistance gene are the limited target spectrum and the potential for rapid adaptation of the pest. Hybrid toxins with a wider target sp...

journal_title：Plant biotechnology journal

authors： Naimov S,Dukiandjiev S,de Maagd RA

更新日期：2003-01-01 00:00:00